Activités au sein de l’équipe « Transferts Ioniques et Rythmicité Cellulaire ».

Participation aux travaux de recherche,

Gestion des flux en produits et consommables de laboratoire,

Entretien d’appareillage,

Personnel compétent en radioprotection et référent OGM de l’équipe TIRC,

Accompagnement technique de doctorants et d’étudiants en Master Recherche,

Intervention en travaux pratiques.

Compétences.

Biologie moléculaire :   –    isolement et purification d’acides nucléiques,

• amplification génique PCR, RT-PCR, single cell, PCR en temps réel (Taqman®, SYBR Green®),
• extinction de l’expression de gènes (siRNA, shRNA),
• séquençage automatique,
• génie génétique (clonage, optogénétique).

 

Biochimie :                    –    extraction et dosage de protéines cellulaires,

• électrophorèse en SDS-page, western-blot, immunoprécipitation,
• transparisation de tissus biologiques (IDISCO).

 

Biologie cellulaire :        –    réalisation de cultures primaires (fibroblastes, cardiomyocytes, progéniteurs cardiaques humains et murins),

• entretien de lignées cellulaires,
• immunomarquage,
• cytométrie en flux.

 

Publications.

Ayad, O., Magaud, C., Sebille, S., Bescond, J., Mimbimi, C., Cognard, C., Faivre, J.-F., Bois, P., and Chatelier, A. (2018). Functional BKCa channel in human resident cardiac stem cells expressing W8B2. FEBS J. 285, 518–530.

Ayad, O., Al Sayed, Z.R., Sebille, S., Magaud, C., Chapotte-Baldacci, C.-A., Jayle, C., Faivre, J.-F., Gaborit, N., Chatelier, A., and Bois, P. (2020). In vitro differentiation of W8B2+ human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity. Cell. Mol. Biol. Lett. 25, 50.

Cabanas, H., Harnois, T., Magaud, C., Cousin, L., Constantin, B., Bourmeyster, N., and Déliot, N. (2018). Deregulation of calcium homeostasis in Bcr-Abl-dependent chronic myeloid leukemia. Oncotarget 9, 26309–26327.

Carre, G., Ouedraogo, M., Magaud, C., Carreyre, H., Becq, F., Bois, P., Supuran, C.T., Thibaudeau, S., Vandebrouck, C., and Bescond, J. (2015). Vasorelaxation induced by dodoneine is mediated by calcium channels blockade and carbonic anhydrase inhibition on vascular smooth muscle cells. J. Ethnopharmacol. 169, 8–17.

Chapotte-Baldacci, C.-A. (2020). Fine Tuning of Calcium Constitutive Entry by Optogenetically-Controlled Membrane Polarization: Impact on Cell Migration. 20.

Domenichini, F., Terrié, E., Arnault, P., Harnois, T., Magaud, C., Bois, P., Constantin, B., and Coronas, V. (2018). Store-Operated Calcium Entries Control Neural Stem Cell Self-Renewal in the Adult Brain Subventricular Zone. Stem Cells Dayt. Ohio 36, 761–774.

El Chemaly, A., Magaud, C., Patri, S., Jayle, C., Guinamard, R., and Bois, P. (2007). The heart rate-lowering agent ivabradine inhibits the pacemaker current I(f) in human atrial myocytes. J. Cardiovasc. Electrophysiol. 18, 1190–1196.

El Chemaly, A., Norez, C., Magaud, C., Bescond, J., Chatelier, A., Fares, N., Findlay, I., Jayle, C., Becq, F., Faivre, J.-F., et al. (2014). ANO1 contributes to angiotensin-II-activated Ca2+-dependent Cl- current in human atrial fibroblasts. J. Mol. Cell. Cardiol. 68, 12–19.

Fourbon, Y., Guéguinou, M., Félix, R., Constantin, B., Uguen, A., Fromont, G., Lajoie, L., Magaud, C., Lecomte, T., Chamorey, E., et al. (2017). Ca2+ protein alpha 1D of CaV1.3 regulates intracellular calcium concentration and migration of colon cancer cells through a non-canonical activity. Sci. Rep. 7, 14199.

Guinamard, R., Demion, M., Magaud, C., Potreau, D., and Bois, P. (2006). Functional expression of the TRPM4 cationic current in ventricular cardiomyocytes from spontaneously hypertensive rats. Hypertens. Dallas Tex 1979 48, 587–594.

Harisseh, R., Chatelier, A., Magaud, C., Déliot, N., and Constantin, B. (2013). Involvement of TRPV2 and SOCE in calcium influx disorder in DMD primary human myotubes with a specific contribution of α1-syntrophin and PLC/PKC in SOCE regulation. Am. J. Physiol. Cell Physiol. 304, C881-894.

Marchand, E., Constantin, B., Balghi, H., Claudepierre, M.-C., Cantereau, A., Magaud, C., Mouzou, A., Raymond, G., Braun, S., and Cognard, C. (2004). Improvement of calcium handling and changes in calcium-release properties after mini- or full-length dystrophin forced expression in cultured skeletal myotubes. Exp. Cell Res. 297, 363–379.

Mok, E., Constantin, B., Favreau, F., Neveux, N., Magaud, C., Delwail, A., and Hankard, R. (2008). l-Glutamine administration reduces oxidized glutathione and MAP kinase signaling in dystrophic muscle of mdx mice. Pediatr. Res. 63, 268–273.

Moubarak, M., Magaud, C., Saliba, Y., Chatelier, A., Bois, P., Faivre, J.-F., and Fares, N. (2015). Effects of atrial natriuretic peptide on rat ventricular fibroblasts during differentiation into myofibroblasts. Physiol. Res. 64, 495–503.

Sabourin, J., Lamiche, C., Vandebrouck, A., Magaud, C., Rivet, J., Cognard, C., Bourmeyster, N., and Constantin, B. (2009). Regulation of TRPC1 and TRPC4 cation channels requires an alpha1-syntrophin-dependent complex in skeletal mouse myotubes. J. Biol. Chem. 284, 36248–36261.

Sabourin, J., Harisseh, R., Harnois, T., Magaud, C., Bourmeyster, N., Déliot, N., and Constantin, B. (2012). Dystrophin/α1-syntrophin scaffold regulated PLC/PKC-dependent store-operated calcium entry in myotubes. Cell Calcium 52, 445–456.

Simard, C., Magaud, C., Adjlane, R., Dupas, Q., Sallé, L., Manrique, A., Bois, P., Faivre, J.-F., and Guinamard, R. (2020). TRPM4 non-selective cation channel in human atrial fibroblast growth. Pflugers Arch. 472, 1719–1732.